Fig. 4: Kdm6b regulates the expression of molecules related to gut homing.

a RT-qPCR analysis for the expression of S1pr1 in the IELPs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice. b Flow cytometry analysis for the expression of S1pr1 in the IELPs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice (n = 5). c IELPs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were stained with anti-CD103 and evaluated by flow cytometry. Statistical results are shown on the right (n = 5). d Geometric mean fluorescence intensity (MFI) of CD103 in CD103⁺ IELPs from indicated mice (n = 5). e RT-qPCR analysis for the expression of CD103 in total IELPs of indicated mice. f, g IELPs and TCRαβ⁺CD8αα⁺ IELs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were stained with anti-α4β7 and evaluated by flow cytometry. Representative result from three independent experiments were shown (n = 5). h TCRαβ⁺CD8αα⁺ IELs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were stained with anti-CD103 and evaluated by flow cytometry. Statistical results are shown on the right (n = 6). i RT-qPCR analysis for the expression of CD103 in TCRαβ⁺CD8αα⁺ IELs of indicated mice. j TCRαβ⁺CD8αα⁺ IELs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were stained with anti-Ccr9 and evaluated by flow cytometry (n = 6). k RT-qPCR analysis for the expression of Ccr9 in TCRαβ⁺CD8αα⁺ IELs of indicated mice. l ChIP-qPCR analysis of H3K27Me3 binding at the promoter of Ccr9 in TCRαβ⁺CD8αα⁺ IELs from indicated mice. m Expression levels of CD8α were evaluated by flow cytometry (n = 5). n RT-qPCR analysis for the expression of CD8a in TCRαβ⁺CD8αα⁺ IELs of indicated mice. o ChIP-qPCR analysis of H3K27Me3 binding at the promoter and E8I enhancer of CD8a in TCRαβ⁺CD8αα⁺ IELs from indicated mice.