Fig. 7: Kdm6b is required for the maturation of TCRαβ⁺CD8αα⁺ IEL.

a TCRαβ⁺CD8αα⁺ IELs in Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were analyzed for their expression of Thy1.2. Statistical results for the percentages of Thy1.2⁺TCRαβ⁺CD8αα⁺ IELs are shown on the right (n = 10). b TCRαβ⁺CD8αα⁺ IELs in Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were analyzed for their expression of Thy1.2 and Gzmb. Statistical results are shown on the right (n = 6). c TCRαβ⁺CD8αα⁺ IELs of wild-type C57BL/6 mice were sorted and stimulated with anti-CD3 plus anti-CD28, IL-2 or IL-15. The expression of indicated genes were analyzed by RT-qPCR. d Sorted TCRαβ⁺CD8αα⁺ IELs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice were stimulated with IL-15 for 4 h and RT-qPCR was performed to detect the expression of indicated genes. e ChIP-qPCR analysis of H3K27Me3 binding at the promoter of indicated genes in TCRαβ⁺CD8αα⁺ IELs from Kdm6b^F/F-CD4Cre and Kdm6b^F/F mice.