Fig. 2: STING promotes retina inflammation upon oxidative injury. | Cell Death & Differentiation

Fig. 2: STING promotes retina inflammation upon oxidative injury.

From: Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration

Fig. 2

A HE staining of mouse retina. Subretinal bleeding was observed in retinas from 4 out of 6 diABZI-injected mice. Scale bar: 100 μM. B IF analysis showing IBA1-positive MPs. Note that diABZI alone increased MP infiltration around the optic nerve. Right panel: quantification of IBA1-positive cells. Scale bar: 200 μM. WB (C) or qRT-PCR (D) showing indicated retinal proteins or gene expression. WB (E) or qRT-PCR (F) showing indicated retinal proteins or gene expression. G HE staining of mouse retina. Arrows indicate retina disorganization and arrow heads indicate swelling and bundling of RPE cells. Scale bar: 200 μM. H IF analysis showing IBA1-positive MPs in retina. Arrows: destructive retina, arrow heads: subretinal localization of MPs. n = 4. Scale bar: 200 μM. I IF analysis shows IBA1-positive MPs in RPE flat mounts. n = 4. Scale bar: 500 μM. Right panel: IBA1-positive cell number in four randomly selected regions. J WB showing indicated proteins in ARPE-19 cells. Cells were exposed to 600 μM H2O2 for 2 h and recovered for 2 h before analysis.

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