Table 2 Primers used for qRT-PCR, qCHIP, MNase assay and mitochondria and nuclear DNA detection.
Primer for specific gene | Primer direction | Primer sequence |
|---|---|---|
Human IL1β | F | CTCGCCAGTGAAATGATGGCT |
Human IL1β | R | GTCGGAGATTCGTAGCTGGAT |
Human IL6 | F | GTGAAAGCAGCAAAGAGGC |
Human IL6 | R | TTTCACCAGGCAAGTCTCC |
Human IL8 | F | TTGGCAGCCTTCCTGATTT |
Human IL8 | R | AACTTCTCCACAACCCTCTGC |
Human IFNB | F | GCTCTCCTGTTGTGCTTCTCC |
Human IFNB | R | CCTCCTTCTGGAACTGCTGC |
Human cGAS | F | ACGTGCTGTGAAAACAAAGAAG |
Human cGAS | R | GTCCCACTGACTGTCTTGAGG |
Human STING | F | ATATCTGCGGCTGATCCTGC |
Human STING | R | GGTCTGCTGGGGCAGTTTAT |
Human GAPDH | F | GAAGGTGAAGGTCGGAGT |
Human GAPDH | R | GAAGATGGTGATGGGATTTC |
Mouse IL1β | F | TGCAGACTCAAACTCC |
Mouse IL1β | R | TGAAAGACGGCACACC |
Mouse IL6 | F | GTTCTCTGGGAAATCGTGG |
Mouse IL6 | R | CTGCAAGTGCATCATCGTT |
Mouse IFNB | F | CTCCAGCTCCAAGAAAGGAC |
Mouse IFNB | R | TGGCAAAGGCAGTGTAACTC |
Mouse cGAS | F | GGAAGGAACCGGACAAGCTA |
Mouse cGAS | R | AACTCCGACTCCCGTTTCTG |
Mouse STING | F | GGAACACCGGTCTAGGAAGC |
Mouse STING | R | TGGATCCTTTGCCACCCAAA |
Mouse mitochondria | F | CTAGAAACCCCGAAACCAAA |
Mouse mitochondria | R | CCAGCTATCACCAAGCTCGT |
Mouse B2M | F | ATGGGAAGCCGAACATACTG |
Mouse B2M | R | CAGTCTCAGTGGGGGTGAAT |
Mouse ACTIN | F | CCTAAGGCCAACCGTGAAAA |
Mouse ACTIN | R | CAGAGGCATACAGGGACAGC |
Mouse GAPDH promoter | F | CCACTTGTGGCAAGAGGCTA |
Mouse GAPDH promoter | R | GTGGAGAGTTGGGACGTGAG |
Mouse cGAS (MNase)#1 | F | AGGAGCAAAATTCACTGCGA |
Mouse cGAS (MNase)#1 | R | CCCACAGGTGATGCTAAGAG |
Mouse cGAS (MNase)#2 | F | TGGAATAGGCATGAGCATCG |
Mouse cGAS (MNase)#2 | R | GTCGCAGTGAATTTTGCTCC |
Mouse cGAS (MNase)#3 | F | TCGGTGTCTTTTTATTCAGGCT |
Mouse cGAS (MNase)#3 | R | TGCAATCCTGTGTGTCCCTT |
Mouse cGAS (MNase)#4 | F | TTGGCTGCTGAGATTCCGTA |
Mouse cGAS (MNase)#4 | R | GCAAAATGAGTTCCGCCAAG |
Mouse cGAS (MNase)#5 | F | GGTTTACAGTGAGTCCCAGGAC |
Mouse cGAS (MNase)#5 | R | TGGCTAGATTTGCCGCCTAC |
Mouse STING (MNase)#1 | F | CGTTTAAAGAGCCAGGCAGTG |
Mouse STING (MNase)#1 | R | TGGATTGTGGTCTGCACGTT |
Mouse STING (MNase)#2 | F | CAGATGGCTAGCAGGGAAGAG |
Mouse STING (MNase)#2 | R | GGAGGGCACCGGACAATTTAT |
Mouse STING (MNase)#3 | F | TTTCGGGGAAATAACCACGC |
Mouse STING (MNase)#3 | R | GGACCTGGACTTCCCTTCAT |
Mouse STING (MNase)#4 | F | GGCGTGGTTATTTCCCCGAA |
Mouse STING (MNase)#4 | R | GGGGAGGGGTTAGACAGGAG |
Mouse STING (MNase)#5 | F | GCTTTGGCAGGAAACACCAAA |
Mouse STING (MNase)#5 | R | AACTGCAACTCAGCTCGCTT |
Mouse STING (ChIP)#1 | F | GCCAGATGGCTAGCAGGGAA |
Mouse STING (ChIP)#1 | R | TGGGTATCAGGGATCCAACAC |
Mouse STING (ChIP)#2 | F | TTTCGGGGAAATAACCACGC |
Mouse STING (ChIP)#2 | R | GGACCTGGACTTCCCTTCAT |
Mouse STING (ChIP)#3 | F | GGCGTGGTTATTTCCCCGAA |
Mouse STING (ChIP)#3 | R | GGGGAGGGGTTAGACAGGAG |
Mouse STING (ChIP)#4 | F | GCTTTGGCAGGAAACACCAAA |
Mouse STING (ChIP)#4 | R | AACTGCAACTCAGCTCGCTT |
Human ISG15 | F | AATGCGACGAACCTC |
Human ISG15 | R | GCTCACTTGCTGCTT |
Human NCF2 | F | ACTGCCTGACTCTGTGGT |
Human NCF2 | R | ACTTGGCTGCCTTTCTTA |
Human IRF7 | F | TACCTGTCACCCTCCCC |
Human IRF7 | R | GTCCCACCACCTTCTGC |
Human KLF4 | F | CTGAGCGGGCGAATTTCCATC |
Human KLF4 | R | CGGGCTGCGGCAAAACCTACA |
Human ZC3HAV1 | F | GATGGACAGAAAGGTG |
Human ZC3HAV1 | R | CGATGTGAAGAAGGAG |
Human RSAD2 | F | CGGAACAGATCAAAGCACT |
Human RSAD2 | R | TTAGATTCAGGCACCAAGC |
Human ACTIN | F | TCACCAACTGGGACGACAT |
Human ACTIN | R | ATCTGGGTCATCTTCTCGC |
Human TFAM | F | CGCTCCCCCTTCAGTTTTGT |
Human TFAM | R | CCAACGCTGGGCAATTCTTC |
Human BRD4 | F | TGCTGACGTCCGATTGATGT |
Human BRD4 | R | TCGAACACATCCTGGAGCTTG |
Human Trp53bp1 | F | CAAAGAATTCTGGACTGGCAACCC |
Human Trp53bp1 | R | TCCAGGAAGTTCTGCTGTTGGTC |
Human BRD4 (shRNA)#1 | F | CCGGCAGTGACAGTTCGACTGATGACTCGAGTCATCAGTCGAACTGTCACTGTTTTTG |
Human BRD4 (shRNA)#1 | R | AATTCAAAAACAGTGACAGTTCGACTGATGACTCGAGTCATCAGTCGAACTGTCACTG |
Human BRD4 (shRNA)#2 | F | CCGGCCTGGAGATGACATAGTCTTACTCGAGTAAGACTATGTCATCTCCAGGTTTTTG |
Human BRD4 (shRNA)#2 | R | AATTCAAAAACCTGGAGATGACATAGTCTTACTCGAGTAAGACTATGTCATCTCCAGG |
Human Trp53bp1 (shRNA)#1 | F | CCGGCCCTTGTTCAGGACAGTCTTTCTCGAGAAAGACTGTCCTGAACAAGGGTTTTTG |
Human Trp53bp1 (shRNA)#1 | R | AATTCAAAAACCCTTGTTCAGGACAGTCTTTCTCGAGAAAGACTGTCCTGAACAAGGG |
Human Trp53bp1 (shRNA)#2 | F | CCGGGATACTCCTTGCCTGATAATTCTCGAGAATTATCAGGCAAGGAGTATCTTTTTG |
Human Trp53bp1 (shRNA)#2 | R | AATTCAAAAAGATACTCCTTGCCTGATAATTCTCGAGAATTATCAGGCAAGGAGTATC |
Human NC (shRNA) | F | CCGGAAGCTGGAGTACAACTACAACCTCGAGGTTGTAGTTGTACTCCAGCTTTTTTTG |
Human NC (shRNA) | R | AATTCAAAAAAAGCTGGAGTACAACTACAACCTCGAGGTTGTAGTTGTACTCCAGCTT |
