Fig. 1: SIRT7 overexpression predicts a poor prognosis in pancreatic cancer and is crucial for pancreatic cancer cell proliferation and progression in vitro and in vivo.
A, B Representative IHC staining and statistical analysis of SIRT7 from 56 pairs of pancreatic tumour and normal adjacent tissues. Scale bar as shown. C Kaplan–Meier overall survival curves for all 56 patients with pancreatic cancer stratified by high and low SIRT7 expression. D Western blotting of SIRT7 expression in HEK293T cells, normal pancreatic ductal epithelial cells (hTERT-HPNE) and pancreatic cancer cell lines. One representative experiment of n = 3 independent experiments is shown. E Western blotting of SIRT7 expression in PANC-1 cells transfected with shRNAs. One representative experiment of n = 3 independent experiments is shown. F MTT assays of PANC-1 cells transfected with shNC, shSIRT7-1 or shSIRT7-2 plasmids. One representative experiment of n = 3 independent experiments is shown. G, H Colony formation assays and statistical analysis of the above groups of PANC-1 cells. One representative experiment of n = 3 independent experiments is shown. I–K The Effects of SIRT7 on tumour xenografts in nude mice. PANC-1 cells with stable SIRT7 silencing by shRNA (shNC, shSIRT7-1 or shSIRT7-2) were injected subcutaneously into the axillae of nude mice (n = 5 for each group). Mice were sacrificed after 4 weeks, and their tumour masses were excised and weighed. Vtumour = 0.5 × L × W2. One representative experiment of n = 3 independent experiments is shown. (The data are shown as the means ± SD. Statistical significance was determined by two-tailed t-tests (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS, no significance)).
