Fig. 4: OGT promotes the stability of SIRT7 via preventing its interaction with REGγ. | Cell Death & Differentiation

Fig. 4: OGT promotes the stability of SIRT7 via preventing its interaction with REGγ.

From: O-GlcNAcylation and stablization of SIRT7 promote pancreatic cancer progression by blocking the SIRT7-REGγ interaction

Fig. 4

A PANC-1 and MiaPaCa-2 cells transfected with MYC-OGT, MYC-OGT-K908A and HA-Ub were used for immunoprecipitation with Protein A/G agarose incubated with anti-SIRT7 antibody, followed by HA western blotting. One representative experiment of n = 3 independent experiments is shown. B PANC-1 and MiaPaCa-2 cells treated with NC/siOGT and HA-Ub were immunoprecipitated with Protein A/G agarose incubated with anti-SIRT7 antibody, followed by HA western blotting. One representative experiment of n = 3 independent experiments is shown. C PANC-1 and MiaPaCa-2c ells transfected with MYC-OGT, HA-Ub, HA-Ub K48, and HA-Ub K63 were immunoprecipitated with Protein A/G agarose incubated with anti-SIRT7 antibody, followed by HA western blotting. One representative experiment of n = 3 independent experiments is shown. D PANC-1 and MiaPaCa-2 cells transfected with MYC-OGT, HA-Ub, HA-Ub K48R, and HA-Ub K63R were immunoprecipitated with immunoprecipitated with Protein A/G agarose incubated with anti-SIRT7 antibody, followed by HA western blotting. One representative experiment of n = 3 independent experiments is shown. E The relationship between SIRT7/REGγ interaction and OGT was detected. PANC-1 and MiaPaCa-2 cells transfected with HA-OGT were immunoprecipitated with Protein A/G agarose incubated with anti-SIRT7 antibody, followed by western blotting for SIRT7 and REGγ. One representative experiment of n = 3 independent experiments is shown. F The relationship between SIRT7/REGγ interaction and O-GlcNAcylation was detected. PANC-1 and MiaPaCa-2 cells treated with PugNAc (1 µM) were immunoprecipitated with Protein A/G agarose and incubated with anti-SIRT7 antibody, followed by western blotting for SIRT7 and REGγ. One representative experiment of n = 3 independent experiments is shown. G The relationship between REGγ and OGT in SIRT7 expression was detected. PANC-1 and MiaPaCa-2 cells were transfected with OGT and siREGγ as indicated. One representative experiment of n = 3 independent experiments is shown.

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