Fig. 6: USP14 promoted pancreatic cancer progression in a TAZ signalling-dependent manner. | Cell Death & Differentiation

Fig. 6: USP14 promoted pancreatic cancer progression in a TAZ signalling-dependent manner.

From: A self-amplifying USP14-TAZ loop drives the progression and liver metastasis of pancreatic ductal adenocarcinoma

Fig. 6

A SW1990 cells with stable overexpression of USP14 and knockdown of TAZ were implanted subcutaneously into the right axillae of nude mice. After 30 days, ectopic xenograft tumours were excised and photographed (n = 6). B Tumour weights were shown (n = 6). C Tumour volumes were measured at 2-day intervals, and growth curves of xenograft tumours were presented. The data were analysed by one-way ANOVA and were presented as the mean ± SD values (n = 6). D H&E staining and IHC (Ki-67 and TAZ) were performed on xenograft tumours originating from SW1990 cells with stable overexpression of USP14 and knockdown of TAZ. Representative IHC image s were shown (n = 6). E, F The percentage of Ki67-positive cells and the relative staining intensity of TAZ were quantified. Scale bar, 100 µm (n = 6). G SW1990 cells with stable overexpression of USP14 and knockdown of TAZ were injected into the spleens of nude mice. After 30 days, the livers of the mice were excised and photographed, and a representative liver was shown. The black arrows indicated liver metastatic nodules. H&E staining showed liver metastatic nodules (n = 10). H The number of liver metastatic nodules was determined and analysed by Student’s t-test (n = 4). I Kaplan-Meier survival analysis was performed to evaluate the OS of mice (n = 6).

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