Fig. 3: Enhanced ferroptosis resulted in radiosensitization of HCC. | Cell Death & Differentiation

Fig. 3: Enhanced ferroptosis resulted in radiosensitization of HCC.

From: SOCS2-enhanced ubiquitination of SLC7A11 promotes ferroptosis and radiosensitization in hepatocellular carcinoma

Fig. 3: Enhanced ferroptosis resulted in radiosensitization of HCC.The alternative text for this image may have been generated using AI.

A Representative fluorescence images of SLC7A11 and GPX4 proteins assessed by immunofluorescence assay in HCC clinical tissues from 12 radioresistant (HCC-R) and 12 radiosensitive (HCC-S) patients. Nuclei were stained with DAPI (×10, ×40). Scale bars, 50 μm. B Western blot assay of SLC7A11, GPX4 and tubulin proteins in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 4 h after 4 Gy IR or non-IR. Representative images (C) and quantification (D) of the relative fluorescence intensity of liperfluo (a lipid peroxide fluorescent probe) in SK-Hep-1, SK-Hep-1R, HepG2 and HepG2R cells at 4 h after 4 Gy IR or non-IR. Nuclei were stained with Hoechst (×40). Scale bars, 10 μm. E Dose responses of survival factions of SK-Hep-1 and HepG2 cells treated with RSL3, and the western blot assay of GPX4 and tubulin proteins in these cells. SK refers to SK-Hep-1, HG refers to HepG2. F, G Western blot assay of GPX4 and tubulin proteins in HCC cells treated with RSL3, siSOCS2 or its control siNC. H, I Dose responses of survival factions of SK-Hep-1 and HepG2 cells treated with RSL3, siSOCS2 or its control siNC. *P < 0.05, **P < 0.01 and ***P < 0.001 between indicated groups.

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