Fig. 7: SOCS2 mediated K48-linked polyubiquitination chains onto SLC7A11 via an elongin B/C ubiquitin-complex. | Cell Death & Differentiation

Fig. 7: SOCS2 mediated K48-linked polyubiquitination chains onto SLC7A11 via an elongin B/C ubiquitin-complex.

From: SOCS2-enhanced ubiquitination of SLC7A11 promotes ferroptosis and radiosensitization in hepatocellular carcinoma

Fig. 7: SOCS2 mediated K48-linked polyubiquitination chains onto SLC7A11 via an elongin B/C ubiquitin-complex.The alternative text for this image may have been generated using AI.

A Immunoprecipitation and immunoblotting assay were selected to detect the type of polyubiquitination of Flag-SLC7A11 in SK-Hep-1 and HepG2 cells transfected with wild-type or K48/K63 mutant Ub after IR. B Western blot assay of SLC7A11, elongin B and elongin C proteins in 4 Gy irradiated SK-Hep-1 and HepG2 cells transfected with siRNA targeting elongin B or elongin C (siEB/C). C Amino acid sequences of SOCS2-BOX regions of SOCS1 (1), SOCS2 (2), SOCS6 (6) and SAB15 (15) proteins from different species. H refers to Human; C refers to Chicken; A refers to African clawed frog; R refers to Red flour beetle. D Schematic representation of two flag-fused SOCS2 plasmids with wild-type (SWT) or L162C166/P162P166 (SLC→PP) mutant. E Co-immunoprecipitation assay of SWT, SLC→PP, elongin B and elongin C in the whole cell lysates of irradiated SK-Hep-1 and HepG2 cells. F SLC7A11 polyubiquitination was detected by anti-Ub immunoblotting assay in 4 Gy irradiated SK-Hep-1 and HepG2 cells transfected with SWT or SLC→PP plasmid.

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