Fig. 3: Erythroid terminal differentiation dependent on caspase-10 activity.
From: Role of Caspase-10-P13tBID axis in erythropoiesis regulation
a The graph shows the percentage of KITlowGPAhigh cells at day 8 of the CD36 culture in the presence of the caspase-10 inhibitor AEVD-Fmk (N = 3). The mean ± SD of replicates is shown, together with the p-value in an unpaired two-tailed Student’s t-test. b Representative western blot detection of caspase-10 performed at day 7 of the CD36 GFP + sorted cells transduced at day 2 with indicated shRNA (N = 8). c Representative plots of the erythroid differentiation of transduced shCASP10-GFP + and control cells as a function of KIT/GPA and of CD49d/Band3 on day 8 of the CD36 culture. The percentage of more mature KITlowGPAhigh and CD49dlowBand3high cells in the gate are indicated. The graph on the right panel shows the percentage of mature cells on day 8 in each experiment. The mean ± SD of eight replicates for sh1CASP10 and three replicates for sh2CASP10 are shown, together with the p-value in an unpaired two-tailed Student’s t-test. d Representative images of May-Grünwald-Giemsa staining of cytospins obtained on day 7 for shCTL and both shCASP10-GFP + cells, when transduction was performed on day 2 of the CD36 culture.
