Fig. 3: T166 A/D mutation regulates the CDDO mediated beigeing program in SAT. | Cell Death & Differentiation

Fig. 3: T166 A/D mutation regulates the CDDO mediated beigeing program in SAT.

From: Blockage of PPARγ T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions

Fig. 3

a 8-week-old WT/WT, TA/TA and TD/TD mice treated with vehicle or CDDO (3 mg/kg) for 14 days. Three kinds of adipose fat pads (percentage of body weight) from WT/WT, TA/TA and TD/TD mice. (Vehicle WT/WT group n = 6; Vehicle TA/TA group n = 6; Vehicle TD/TD group n = 6; CDDO WT/WT group n = 8; CDDO TA/TA group n = 8; CDDO TD/TD group n = 8). b Hematoxylin and eosin (H&E) staining of SAT. 100× magnification, scale bar, 100 μm. (n = 5) (c) Quantitation of the size and percentage of lipid droplets in SAT by TissueFAXS Cytometry analysis. (n = 5) (d) UCP1 immunohistochemical staining of SAT. 200× magnification, scale bar, 50 μm. (n = 5) (e) Relative mRNA levels of browning-related genes in isolated adipocytes from SAT. (Vehicle WT/WT group n = 6; Vehicle TA/TA group n = 6; Vehicle TD/TD group n = 6; CDDO WT/WT group n = 8; CDDO TA/TA group n = 8; CDDO TD/TD group n = 8). Data are expressed as the mean ± S.E.M. Data were analyzed by one-way ANOVA followed by Tukey’s test (a, c, e). *P < 0.05, **P < 0.01, ***P < 0.001.

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