Fig. 1: Epithelial NEMO ablation delays tumor onset and prolongs survival in a mouse model of SCLC.
From: NEMO- and RelA-dependent NF-κB signaling promotes small cell lung cancer
A Schematic showing intratracheal Ad-Cre inhalation and experimental procedure for the generation and analysis of a mouse model of SCLC (created with BioRender.com)B Representative images of MRI scans of Rb1FL/FL Tp53FL/FL (n = 17) and Rb1FL/FL Tp53FL/FL NemoFL/FL (n = 9) mice 26 and 32 weeks after Ad-Cre inhalation. Tumor areas were marked using Horos Software. C Graph depicting tumor onset assessed via MR imaging of mice with indicated genotypes. ****p < 0.0001, Log-rank test. D Graph depicting the number of tumor lesions 6 weeks after Ad-Cre inhalation of the indicated genotypes. ***p = 0.001, Mann–Whitney Test. Mean ± SEM. Each dot represents one mouse. E Graph depicting tumor fold change assessed via MR imaging 2–6 weeks after Ad-Cre inhalation for indicated genotypes. Each dot represents one mouse. Bars represent mean ± SEM. *p < 0.05, Mann–Whitney Test. F Graph depicting survival of mice with indicated genotypes. ****p < 0.0001, Log-rank test. G Representative photographs of lungs of Rb1FL/FL Tp53FL/FL (n = 17) and Rb1FL/FL Tp53FL/FL NemoFL/FL (n = 10) mice sacrificed at the humane endpoint. H Graphs depicting the percentage of mice with liver metastasis and the amount of liver tumor lesions at the humane endpoint. n = 7 for Rb1FL/FL Tp53FL/FL NemoFL/FL, n = 14 for Rb1FL/FL Tp53FL/FL. Mann–Whitney test. Mean ± SEM.
