Fig. 6: IKK2ca expression increased inflammatory gene transcription but did not alter the morphology or immune cell infiltration of SCLC.

A Representative images of lung sections from mice stained with H&E. (Scale bars = 5 mm (top) and 100 µm (bottom)). Samples were taken at the humane endpoint B Representative images of lung sections from mice of the indicated genotypes at the humane endpoint immunostained for CD56. (Scale bars 5 mm (top) and 50 µm (bottom), n = 8 for Rb1^FL/FL Tp53^FL/FL R26^LSL.IKK2ca, n = 11 for Rb1^FL/FL Tp53^FL/FL C Representative images of lung sections from mice of the indicated genotypes at the humane endpoint immunostained for Ki67 and graph showing quantifications of Ki67 positive cells. Mann–Whitney test. Mean ± SEM. (Scale bars 5 mm (top) and 50 µm (bottom), n = 10 for Rb1^FL/FL Tp53^FL/FL R26^LSL.IKK2ca, n = 13 for Rb1^FL/FL Tp53^FL/FL). D Representative images of lung sections from Rb1^FL/FL Tp53^FL/FL R26^LSL.IKK2ca (n = 10) and Rb1^FL/FL Tp53^FL/FL (n = 13) mice at the humane endpoint immunostained for CD45 and graph depicting the amount of CD45 positive cells. Mann–Whitney test. Mean ± SEM. Scale bars = 100 µm (top) and 25 µm (bottom). E–G Graphs depicting PCA E, Gene set enrichment analysis (F) and Volcano plot (G) of RNA seq data from tumor tissues of Rb1^FL/FL Tp53^FL/FL R26^LSL.IKK2ca (n = 6) compared to Rb1^FL/FL Tp53^FL/FL (n = 6) mice. In (G), genes that were found significantly upregulated (p < 0.01, log2(|FC | ) > 2) or downregulated (p < 0.01, log2(|FC | ) < 2) in tumors from Rb1^FL/FL Tp53^FL/FL R26^LSL.IKK2ca compared to Rb1^FL/FL Tp53^FL/FL mice are indicated with red or blue dots, respectively. H Representative immunoblot analysis with the indicated antibodies of protein extracts from tumor tissue derived from RP-mice with indicated genotypes at the humane endpoint. Uncropped images of immunoblots are presented in Supplementary Fig. 1. Data from the same Rb1^FL/FL Tp53^FL/FL cohort are included in all figures for comparison.