Fig. 8: TNFR1 deficiency did not alter SCLC morphology and immune cell infiltration.
From: NEMO- and RelA-dependent NF-κB signaling promotes small cell lung cancer
A Representative images of lung sections from mice at the humane endpoint stained with H&E (Scale bars = 5 mm (top) and 100 µm (bottom)) B or immunostained for CD56 (Scale bars 5 mm (top) and 50 µm (bottom), n = 5 for Rb1FL/FL Tp53FL/FL Tnfr1FL/FL, n = 8 for Rb1FL/FL Tp53FL/FL Tnfr1−/−, n = 11 for Rb1FL/FL Tp53FL/FL C Representative images of lung sections from mice at the humane endpoint immunostained for Ki67 and graph depicting Ki67 positive cells. Kruskal–Wallis test. Mean ± SEM. (Scale bars 2.5 & 5 mm (top) and 50 µm (bottom), n = 10 for Rb1FL/FL Tp53FL/FL Tnfr1FL/FL, n = 10 for Rb1FL/FL Tp53FL/FL Tnfr1−/−; n = 13 for Rb1FL/FL Tp53FL/FL). D Representative images of lung sections immunostained for CD45 and graph depicting the amount of tumor-infiltrating CD45 positive cells. Kruskal–Wallis test. Mean ± SEM. Scale bars = 100 µm (top) and 25 µm (bottom). Samples were taken at the humane endpoint (n = 10 for Rb1FL/FL Tp53FL/FL Tnfr1FL/FL, n = 10 for Rb1FL/FL Tp53FL/FL Tnfr1−/−, n = 13 for Rb1FL/FL Tp53FL/FL). E, F PCA E and Volcano plot F of RNA seq data from tumor tissues of Rb1FL/FL Tp53FL/FL Tnfr1FL/FL (n = 5) and Rb1FL/FL Tp53FL/FL Tnfr1−/− (n = 5) mice compared to tumors from Rb1FL/FL Tp53FL/FL (n = 6) mice. Genes that were found significantly upregulated (p < 0.01, log2(|FC | ) > 2) or downregulated (p < 0.01, log2(|FC | ) < 2) in these comparisons are indicated with red or blue dots, respectively. Data from the same Rb1FL/FL Tp53FL/FL cohort are included in all figures for comparison.
