Fig. 2: MARVELD1 interacts with DDR proteins and is a DDR mediator. | Cell Death & Differentiation

Fig. 2: MARVELD1 interacts with DDR proteins and is a DDR mediator.

From: The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells

Fig. 2

a Lysates of HU treatment HeLa/PC or HeLa/MARVELD1-Flag cells were immunoprecipitated with anti-Flag antibody. The gel pieces containing regions of interest (area shown in the bracket) were analyzed by mass spectrometry. Venn diagram showing the number of MARVELD1 interaction proteins. b The difference of protein–protein interaction (PPI) was obtained between the control and HU group. c GO analysis were performed for the MARVELD1-interacting proteins. d DDR-related GO terms and their correlated MARVELD1 interactors were constructed into a DDR-related function-protein network (the green circles mark the sensor proteins). The dot size indicates more unique peptides or a higher ratio of HU/Ctrl. e Schematic diagram show that MARVELD1 and PARP1 share interacting protein families of MCM and YWHA (right), along with their related functions (left). f MARVELD1 and PARP1 interactors were confirmed by Co-immunoprecipitation (Co-IP) and Western blotting (WB) in HeLa cells (the red squares mark the interactions after HU treatment). g MARVELD1 knockdown whether affect the interaction between PARP1 and DDR proteins was confirmed by Co-IP and WB in HeLa cells (the red squares mark the interactions).

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