Fig. 4: CuET induces cell death in a p53-independent manner. | Cell Death & Differentiation

Fig. 4: CuET induces cell death in a p53-independent manner.

From: Actionable cancer vulnerability due to translational arrest, p53 aggregation and ribosome biogenesis stress evoked by the disulfiram metabolite CuET

Fig. 4: CuET induces cell death in a p53-independent manner.The alt text for this image may have been generated using AI.

A. Representative light microscope images following treatment of A549 or U2OS cells with various concentrations of CuET for different time points. Scale bar: 10 μM. B CuET dose-response curves in A549 and U2OS cells. GI50 values are shown in the table with 95% confidence intervals, along with R2 values quantifying the goodness of fit (data are shown as mean ± SD, n = 3 biological replicates). C Cell survival analysis (resazurin) in A549 cells treated with siRNA against TP53 +/− various concentrations of CuET for 24 h. D Quantitative RT-PCR of various p53 targets in A549 cells treated with CuET (6 h) +/− siRNA against TP53 (data are shown as mean ± SD, n = 3 biological replicates, **p < 0.01, *p < 0.05, non-significant values are not shown). E Barplot of pathway enrichment terms (Reactome) for genes that are monotonically up-or down-regulated following CuET treatment. F Scatterplot depicting the correlation of Reactome terms among DE genes affected by CuET or thapsigargin.

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