Fig. 1: SENP3 is downregulated in PDAC tissues and correlated with a favorable prognosis.

A Schema of an in vivo screening strategy for identifying SENPs that modulate PDAC metastasis. Lentivirus particles targeting each SENP family member were individually produced in HEK293T cells and subsequently infected into GFP and luciferase-producing PDAC cell lines (PANC-1 and Patu-8988t). Infected cells were selected with puromycin and then mixed (for each SENP-targeted cell line 3 × 10⁴ cells were employed) for orthotopic injection into pancreas of ten nude mice for each cell line. Eight weeks later, vigorous metastatic liver nodules detected with the IVIS imaging system were isolated from tumor-bearing mice. GFP-positive metastatic cells were isolated with FACS sorting following single-cell suspension collection by liver digestion. Genomic DNA extracted from sorted cells was amplified and verified by DNA sequencing. B Representative IHC image of SENP3 in PDAC and paired peri-tumor tissues using PDAC tissue microarray(TMA), Scale bars shown is 200 μm. C Statistical analysis of IHC scores from TMA was performed, unpaired t test. ****p < 0.0001. D Expression of SENP3 was determined by western blot analysis in ten paired primary pancreatic cancer tissues (T) and the matched adjacent nontumor tissues (N) from our center. E Overall survival based on SENP3 expression in TAM. log-rank test.