Fig. 3: Wild-type SENP3 inhibits PDAC metastasis.
From: SUMO specific peptidase 3 halts pancreatic ductal adenocarcinoma metastasis via deSUMOylating DKC1
A, B Transwell assay detected the migration ability after endogenous overexpression of wild-type SENP3 (wt-oe) or enzymatically defective SENP3 mutants (C532S, mut-oe) in Patu-8988t cells and PANC-1 cells. C, D Wound healing assay analyzed the migration ability of Patu-8988t cells infected with indicated overexpression vectors. Statistical analysis was performed according to scratch width at 48 h. E, F The in vivo migration potency of vector control, wild-type SENP3, C532S SENP3-expressing Patu-8988t cells. Luciferase-expressing Patu-8988t cells were subjected to lentiviral infection to express either vector control (vector), wild-type SENP3 (wt-oe) or catalytically deficient C532S SENP3 (mut-oe) and then injected into pancreases of mice, luciferase signal was monitored weekly, images in E was detected at eight weeks after inoculation. Relative luciferase signal of metastatic signal (F) was calculated using relative metastatic signal/ orthotopic signal. Unpaired t test, no significance, *p < 0.05, **p < 0.01.
