Fig. 4: Curcumin-induced NRF2 directly transactivates miR-34a and miR-34b/c.
From: Curcumin activates a ROS/KEAP1/NRF2/miR-34a/b/c cascade to suppress colorectal cancer metastasis
A, B qPCR analysis of A. pri-miR-34a and B. pri-miR-34b/c expression in HCT116 cells treated as indicated for 48 hours. C qPCR analysis of indicated mRNAs in SW480 cells after transfection with empty pcDNA3.1 or NRF2 pcDNA3.1 vectors for 72 hours. D Western blot analysis of NRF2 and NQO1 proteins in SW480 cells after transfection with pcDNA3.1 and NRF2 pcDNA3.1 for 72 hours. E ARE consensus sequence defined as 5ʹ-A/G TGA C/G NNNGC A/G-3ʹ, where “N” represents any nucleotide according to jaspar.genereg.net (MA0150.1). F Map of human miR-34a and miR-34b/c genomic regions with indicated NRF2 binding sites. G Sequences of NRF2 binding sites within the miR-34a and miR-34b/c genomic regions. H qChIP analysis of NRF2 occupancy at the human miR-34a and miR-34b/c genomic regions in HCT116 p53 −/− cells after treatment with curcumin or DMSO for 48 hours. NQO1 and 16q22 served as positive and negative controls, respectively. In (A)–(C), and (H) mean values ± SD are shown (n = 3). **P < 0.01, ***P < 0.001.
