Fig. 7: Curcumin induces MET and inhibits lung-metastases formation via inducing miR-34a.
From: Curcumin activates a ROS/KEAP1/NRF2/miR-34a/b/c cascade to suppress colorectal cancer metastasis
A, B Expression of pri-miR-34a (A) and mature miR-34a (B) in SW620-luc2 cells treated with curcumin or DMSO for 48 hours. C Analysis of SW620-luc2 cell migration after transfection with miR-34 antagomirs and/or curcumin for 72 hours. D Analysis of SW620-luc2 cell invasion after transfection with miR-34 antagomirs and/or curcumin for 72 h. E qPCR analyses of EMT-related genes in SW620-luc2 cells 72 hours after the indicated treatments/transfections. F–I Formation of lung metastases by SW620-Luc2 cells, which were treated as indicated for 48 hours and then injected into tail-vein of NOD/SCID mice. Representative images of luciferase signals at the indicated time points after xenografting (F) and the quantification of total photon flux (G). H right: representative lungs 5 weeks after tail vein injection. Arrows indicate metastatic tumor nodules. Left: representative images of H&E-stained resected lungs. Scale bar: 500 μm; 50 μm (insert). I. Quantification of metastatic nodules in the lungs of indicated mice. In (A)–(E) mean values ± SD (n = 3) are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
