Fig. 8: Knockout of B3GNT3 or knockdown SLC3A2 retarded PDAC cell growth and potentiated IKE or TM-mediated PDAC suppression in vitro and in vivo. | Cell Death & Differentiation

Fig. 8: Knockout of B3GNT3 or knockdown SLC3A2 retarded PDAC cell growth and potentiated IKE or TM-mediated PDAC suppression in vitro and in vivo.

From: Targeting N-glycosylation of 4F2hc mediated by glycosyltransferase B3GNT3 sensitizes ferroptosis of pancreatic ductal adenocarcinoma

Fig. 8

A Schematic of the experimental design for nude mice bearing PANC-1 xenografts with the indicated genotypes. B Representative subcutaneous transplant tumors (STTs) were removed and photographed on day 28 (n = 4 mice/group). C Relative tumor volume of STTs. D The tumor growth curves of STTs were drawn and assessed weekly. E Gross appearances of representative orthotopic transplantation tumors (OTTs) were removed and photographed on day 28 (n = 7 mice/group). F Relative tumor volume of OTTs. For detailed methods of STTs and OTTs experiments, see the methods section. G Schematic of the experimental design for nude mice bearing PANC-1 cells stably transfected with shControl and shSLC3A2. H Representative orthotopic transplantation tumors (OTTs) were removed and photographed on day 28 (n = 4 mice/group). I Tumor weight. J Tumor volume. K Left, representative IVIS bioluminescence images on day 7 and day 28; right, representative hematoxylin and eosin (HE)-stained tumor section. Scale bar, 2.5 mm for zoom out and 250 μm for zoom in. N, normal tissue. T, tumor. L Left, representative HE sections with corresponding immunohistochemical staining for Ki-67, Scale bar, 250 μm. M Percentage of Ki-67 positive stained cells per field at least 5 random fields were selected. N Treatment scheme for OTT nude mice bearing shSLC3A2 PANC-1 cells xenograft. BALB/c nude mice were randomly divided into four groups and injected intraperitoneally with the vehicle, 40 mg/kg IKE, 1 mg/kg TM, or the combination of IKE and TM once every other day. O Gross appearances of representative OTT were removed and photographed on day 28 (n = 5 mice/group). P Tumor weight. Q Tumor volume. R Left, representative IVIS bioluminescence images on day 7 and day 28; right, representative HE-stained tumor section. Scale bar, 2.5 mm for zoom out and 250 μm for zoom in. N, normal tissue. T, tumor. S Left, representative HE sections with corresponding immunohistochemical staining for Ki-67, Scale bar, 250 μm. T Percentage of Ki-67 positive stained cells per field at least 5 random fields were selected. All data are shown as the mean ± SD. Statistical significance among the indicated groups was assessed by ANOVA analysis or unpaired Student’s t-test. *P < 0.05, **P < 0.01, ****P < 0.0001.

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