Fig. 4: Comprehensive gene expression analysis of miR-34a/b/c-deficient cells after treatment with 5-FU. | Cell Death & Differentiation

Fig. 4: Comprehensive gene expression analysis of miR-34a/b/c-deficient cells after treatment with 5-FU.

From: CRISPR/Cas9-mediated inactivation of miR-34a and miR-34b/c in HCT116 colorectal cancer cells: comprehensive characterization after exposure to 5-FU reveals EMT and autophagy as key processes regulated by miR-34

Fig. 4

A Principal component analysis of mRNAs expression in WT and miR-34a/b/c-KO HCT116 cells treated with DMSO or 5-FU for 48 h. B Volcano plots showing differential RNA expression with FDR in −log10 scale and fold change in log2 scale. Significantly up- and downregulated genes (FDR < 0.05 & absolute fold change > 1.5) are highlighted in red and blue respectively as indicated. Non-significantly regulated genes are shown in gray. C Venn diagram displaying the number of differentially expressed mRNAs shared between WT and miR-34a/b/c-KO cells (5-FU vs control). D Overrepresentation analysis (ORA) of mRNAs significantly regulated in untreated miR-34a/b/c-KO vs. wild-type cells. E Overrepresentation analysis (ORA) of mRNAs significantly regulated in 5-FU-treated miR-34a/b/c-KO and wild-type cells.

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