Fig. 5: Comprehensive identification of differentially regulated miR-34 targets and their association with functional categories. | Cell Death & Differentiation

Fig. 5: Comprehensive identification of differentially regulated miR-34 targets and their association with functional categories.

From: CRISPR/Cas9-mediated inactivation of miR-34a and miR-34b/c in HCT116 colorectal cancer cells: comprehensive characterization after exposure to 5-FU reveals EMT and autophagy as key processes regulated by miR-34

Fig. 5

A Heatmap of RNA expression of mRNAs with statistically significant, genotype-dependent differences (>1.5-fold) in regulation after 5-FU treatment grouped in the indicated transcriptional clusters. Selected miR-34 targets are indicated by color. Red: published; black: predicted. B Heatmap showing enrichment of the indicated pathways and functional categories in the expression clusters as shown in (A). Statistical significance was calculated by Fisher’s exact test. C Line plot representation of mean z-score of normalized RNA expression of all miR-34 targets with statistically significant, genotype-dependent differences in regulation after 5-FU treatment, grouped in the indicated transcriptional clusters. D Venn diagram displaying the number of shared DREAM, E2F and miR-34 targets in the indicated transcriptional clusters. E Heatmap of GSVA analysis of the indicated pathways activities changes caused by miR-34a/b/c inactivation after 5-FU treatment.

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