Fig. 3: DDR induces premature centriole disengagement.
From: Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis
A HeLa cells expressing GFP-centrin 2 were treated with B[a]P and analyzed by live cell imaging. Arrows point to disengaged centrioles. B–E Disengagement of centrioles in chemically treated HeLa cells was stained with the indicated antibodies and analyzed (n = 300). Ninein served as a marker for the mother centriole (B). F, G After treatment with the indicated DNA damaging agents, RPE-1 cells were stained with the indicated antibodies (F). The number of RPE-1 cells with centriole overduplication or premature centriole disengagement was analyzed as in C and D (G; n = 300 cells). Scale bars, 5 µm. Error bars, SEM. *p < 0.01 (two-tailed t test).
