Fig. 6: Hornerin mediates Plk1 phosphorylation by Chk1.
From: Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis
A–D siRNA-transfected HeLa cells were treated with B[a]P and analyzed by IP-immunoblotting (A and E), immunostaining (B and C; n = 300), and immunoblotting (D). E siRNA-transfected HeLa cells were treated with B[a]P and analyzed by IP-immunoblotting. F Twenty-four hours after transfection of WT or mutant Plk1, the cell lysates were incubated with recombinant His-Plk1 and proteins pulled down with Ni-beads were analyzed by immunoblotting. G HeLa cells transfected with the indicated plasmid were subjected to IP-immunoblotting. H Hornerin-Plk1 3D proximity by in situ PLA in HeLa cells transfected with myc-Plk1 WT or mutants (n = 30). I HeLa cells were stained with hornerin (green), γ-tubulin (red), and DAPI (blue). Images of uncropped blots and gels are provided as a Supplementary Material file. AU, arbitrary units, Scale bars, 5 µm. Error bars, SEM. *p < 0.01 (two-tailed t test).
