Fig. 7: DDR abrogates Plk1 activation and translocation.
From: Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis
A Twenty-four hours after siRNA-transfection, HeLa cells were transfected with the indicated plasmids and analyzed by immunostaining (n = 300). B, C HeLa cells were treated with B[a]P and analyzed by immunostaining (n = 300). D–G HeLa cells transfected with the indicated plasmid were analyzed by immunostaining (n = 300). H Twenty-eight hours after transfection of WT or mutant Plk1, the cells were treated with B[a]P for two hours and then analyzed by immunoblotting. Images of uncropped blots are provided as a Supplementary Material file. KD, kinase domain. AU, arbitrary units, Scale bar, 5 µm. Error bars, SEM. *p < 0.01 (two-tailed t test).
