Fig. 1: APAP induces apoptotic BH3-only proteins but fails to activate caspases.

A Serum ALT of mice treated for 6 h with PBS or 500 mg/kg APAP i.p. Bold dotted line indicates median and weak dotted lines show quartiles. B MTT assay of primary murine (PMH) and human hepatocytes (PHH) treated for 16 h or 24 h, respectively, or FACS analysis of AnnexinV-FITC + IHH and HepG2 cells treated for 24 h. n = 5 (PMH), n = 2 (PHH), n = 13 (IHH), n = 4 (HepG2). C, D Transcript levels of mouse liver of mice treated as described in A, and of PMH, PHH and IHH treated for 3 h with 10 mM APAP (PMH) or with 20 mM APAP (PHH, IHH). n = 7 (PMH), n = 6 (PHH), n = 5 (IHH). E Top 20 gene ontologies of hepatocyte subset from mice treated as described in A generated from single cell RNA sequencing. BP biological function. F Western blot of ML of mice treated as described in A and of PHH and HepG2 treated with 20 mM APAP for 12 h. G Schematic illustration of APAP-induced apoptotic processes. Data points and/or bar graphs are mean +/− SD with n as independent biological replicates. Statistical significance was tested using unpaired Student’s t test (C, D).