Fig. 2: Depletion of BIM protects from APAP-induced liver necrosis.
From: Non-canonical BIM-regulated energy metabolism determines drug-induced liver necrosis
A–C Serum ALT and necrotic area scored from histology images (see Supplementary Fig. S3A) of WT, Noxa−/− and Bim−/− mice treated for 6 h with 500 mg/kg (A, B) or for 24 h with 300 mg/kg APAP (C). Bold dotted line indicates median and weak dotted lines show quartiles. Statistical significance was tested using Two-way ANOVA with Sidak’s multiple comparison test. D Western Blot of BCL-xL immunoprecipitation of primary murine hepatocytes (PMH) untreated (UT) or treated for 3 h with 10 mM APAP or 2 ng/ml murine TNF and 30 nM Actinomycin D (ActD). E Western Blot of cytosolic (C) and mitochondrial (M) fractions of PMH treated with 10 mM APAP for 6 h.
