Fig. 3: BIM contributes to APAP-induced mitochondrial damage and energy crisis.
From: Non-canonical BIM-regulated energy metabolism determines drug-induced liver necrosis
A Energy Map of Seahorse Induced ATP Rate Assay with medium (UT) or APAP injection (final 15 mM APAP) of primary murine hepatocytes (PMH), n = 4–8. Data normalized to sum of mito- and gylcoATP of WT untreated PMH. B Representative confocal images of MitoTracker Green-stained PMH and quantification of mitochondria morphology based on length to width ratio, n = 3. Scale bar 20 µm. C Western Blot of PMH treated with 20 mM APAP for 6 h. D Transcript levels of untreated murine livers (ML) of WT and Bim−/− mice. E, F Western Blot of PMH treated with 20 mM APAP for 6 h (E) and ML of mice treated with 300 mg/kg APAP for 24 h (F). Data points and/or bar graphs are mean +/− SD with n as independent biological replicates. Statistical significance was tested using unpaired Student’s t test (D).
