Fig. 2: KDM5B negatively regulates ZBTB16 expression in NPC. | Cell Death & Differentiation

Fig. 2: KDM5B negatively regulates ZBTB16 expression in NPC.

From: Deubiquitinase USP7 stabilizes KDM5B and promotes tumor progression and cisplatin resistance in nasopharyngeal carcinoma through the ZBTB16/TOP2A axis

Fig. 2

a, b HNE1 cells infected by siControl or siKDM5B were harvested for Transcriptome RNA sequencing. Heatmap (a) and volcano plot (b) were used to show the differential expressed genes. c Venn diagrams showing numbers of target genes of KDM5B and downregulated genes in the GSE118719 and GSE53819 databases. d The bar chart showing the top of 10 genes ranking according to Log2FC value. e Differential expression analyses of ZBTB16 between tumor and normal tissues in GSE53819 databases. f ZBTB16 was negatively correlated with KDM5B in GSE53819 databases. g HNE1 and CNE2 cells were infected with shControl, shKDM5B #1, or shKDM5B #2 for 72 h. Cells were collected for Western blotting analysis and RT-qPCR analysis. h HNE1 and CNE2 cells were infected with indicated plasmids for 72 h. Cells were collected for Western blotting analysis and RT-qPCR analysis. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data presented as Mean ± SD with three replicates. **P < 0.01; ***P < 0.001. i, j The ChIP-seq of KDM5B on the promoter region of ZBTB16. k–m The ChIP-qPCR of KDM5B on the promoter region of ZBTB16 in HNE1 and CNE2 cells (k, l). And the DNA electrophoresis of the products from the ChIP assay (m). Statistical significance was determined by two-side Student t-test. Data presented as Mean ± SD with three replicates. ***P < 0.001.

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