Fig. 4: ZBTB16 negatively regulates TOP2A expression in NPC.

a Venn diagrams showing numbers of target genes of ZBTB16 and upregulated genes in the GSE118719 and GSE53819 databases. b The bar chart showing the top of 10 genes ranking according to Log2FC value. c volcano plot was used to show the differential expressed genes from RNA-seq. d Differential expression analyses of TOP2A between tumor and normal tissues in GSE53819 databases. e TOP2A was negatively correlated with ZBTB16 in GSE53819 databases. f TOP2A was positively correlated with KDM5B in GSE53819 databases. g HNE1 and CNE2 cells were infected with shControl, shZBTB16 #1, or shZBTB16 #2 for 72 h. Cells were collected for Western blotting analysis and RT-qPCR analysis. h HNE1 and CNE2 cells were infected with indicated plasmids for 72 h. Cells were collected for Western blotting analysis and RT-qPCR analysis. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data presented as Mean ± SD with three replicates. **P < 0.01; ***P < 0.001. i, j The ChIP-seq of ZBTB16 on the promoter region of TOP2A. k–o HNE1 cells infected with shControl, shZBTB16, shKDM5B, were harvested for ChIP-qPCR using ZBTB16 antibody (k–m). The relative quantification of ChIP-qPCR in different groups was presented (n). And the DNA electrophoresis of the products from the ChIP assay (o). Statistical significance was determined by two-side Student t-test. Data presented as Mean ± SD with three replicates. NS not significant; *P < 0.05; **P < 0.01. ***P < 0.001.