Fig. 2: Endogenous BAX and BAK together form mosaic rings in cellulo.
From: Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria
A STED image of an untreated, fixed U-2 OS WT cell, immunolabeled for endogenous BAX (green, STED), BAK (magenta, STED) and TOM20 (blue, confocal). B STED image of an apoptotic, fixed U-2 OS WT cell, immunolabeled for endogenous BAX (green, STED), BAK (magenta, STED) and TOM20 (blue, confocal). The cells were treated for 18 h with 10 µM ABT-737, 10 µM Actinomycin D and 20 µM QVD-OPh. C Enlarged insets from (B). (i) Some apoptotic rings are largely comprised of either BAK (wide arrowhead) or BAX (narrow arrowhead). (ii) Most rings contain both BAX and BAK (arrow). D Exemplary STED images showing the great heterogeneity in size and composition of the BAX–BAK rings. Scale bars: 5 µm (A and B), 1 µm (C), 200 nm (D).
