Fig. 1: Genetic blockade of caspase-8-mediated RIPK3 cleavage does not lead to spontaneous necroptosis in vivo.
From: RIPK3 cleavage is dispensable for necroptosis inhibition but restricts NLRP3 inflammasome activation
a WebLogo demonstrating high conservation of the caspase-8-mediated cleavage motif in RIPK3 (mouse numbering) in 94 vertebrate species. b Representative pictures of ~400 days old Ripk3+/+ and Ripk3D333A/D333A mice. c Schematic representation of RIPK3 cleavage fragments. d Western blot analysis of caspase-8-mediated cleavage of RIPK3 in lysates from MDFs treated with 3 h of TS and TSI. NT, non-treated; TS, 100 ng ml−1 TNF (T) plus 1 μM Smac-mimetic compound A (S); TSI, TS plus 10 μM caspase inhibitor IDN-6556 (I). Results are representative of 3 independent experiments performed with 3 independent biological replicates.
