Fig. 2: PRTN3 directly interacts with STAT3.
From: Proteinase 3 depletion attenuates leukemia by promoting myeloid differentiation
a The Co-immunoprecipitation (Co-IP) complex was subjected to silver-staining (n = 3); b the peptide sequences of the STAT3 protein as detected in the Co-IP complex by mass spectrometry; c WB analysis of Co-IP complex confirmed that STAT3-Flag interacted with PRTN3-HA in HEK 293 T cells (n = 3); d WB analysis of Co-IP complex confirmed that PRTN3-HA interacted with STAT3-Flag in HEK 293 T cells (n = 3); e measurements of the binding affinity between STAT3 and PRTN3 by SPR method. f WB analysis of Co-IP complex confirmed that STAT3 interacted with PRTN3 in CD34+ cells (n = 3); g WB analysis of Co-IP complex confirmed that PRTN3 interacted with STAT3 in CD34+ cells (n = 3); h the immunofluorescence showed that STAT3 colocalized with PRTN3 and were expressed in both the cytoplasm of CD34+ cells (n = 3). Data are the mean ± s.d.; n: biologically independent experiments. Statistical analysis was performed using an unpaired two-tailed Student’s t test.
