Fig. 2: DNMT1 deficiency leads to cell number decrease of terminal erythroblasts. | Cell Death & Differentiation

Fig. 2: DNMT1 deficiency leads to cell number decrease of terminal erythroblasts.

From: DNMT1 regulates human erythropoiesis by modulating cell cycle and endoplasmic reticulum stress in a stage-specific manner

Fig. 2: DNMT1 deficiency leads to cell number decrease of terminal erythroblasts.

A qRT-PCR results showing DNMT1 mRNA expression levels in luciferase-shRNA or DNMT1-shRNAs transduced erythroblasts cultured for 7, 11, and 15 days. GAPDH was used as internal reference. B Representative western blot showing DNMT1 protein levels in luciferase-shRNA or DNMT1-shRNAs transduced erythroblasts cultured for 7, 11, and 15 days. C Quantitative analysis of DNMT1 protein levels from 3 independent experiments. D Growth curves of cells transduced with lentivirus containing luciferase-shRNA or DNMT1-shRNAs and N-DMSO or DNMT1 inhibitor cells for 7, 9, 11, 13, and 15 days. E Representative flow cytometry profiles of cell cycle as assessed by Edu and 7AAD staining of cells cultured for 7, 11 days. F, G Quantitative analysis of cell cycle. H Representative flow cytometry profiles of apoptosis as assessed by dual staining of Annexin V+ and 7AAD of cells cultured for 7, 9, 11, 13, and 15 days. I Quantitative analysis of apoptosis from 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

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