Fig. 3: DNMT1 deficiency led to cell cycle arrest due to reduction in methylation level of p21. | Cell Death & Differentiation

Fig. 3: DNMT1 deficiency led to cell cycle arrest due to reduction in methylation level of p21.

From: DNMT1 regulates human erythropoiesis by modulating cell cycle and endoplasmic reticulum stress in a stage-specific manner

Fig. 3

A mRNA levels of p21, p27, p57, p15, p16, p18, and p19, as assessed by qRT-PCR. GAPDH was used as internal reference. B Representative western blot analysis of p21. GAPDH was used as loading control. C Quantitative analysis of p21 from 3 independent experiments. D Bisulfite sequencing of cells. E mRNA levels of p53, as assessed by qRT-PCR. F Representative western blot analysis of p53. GAPDH was used as loading control. G Quantitative analysis of p53 from 3 independent experiments. H Representative flow cytometry profiles of cell cycle as assessed by Edu and 7AAD staining of N-DMSO and 1 μM DC_517 cells cultured for 6 days in the presence of DMSO or 1 μM UC2288. I Quantitative analysis of cell cycle. J Growth curves of N-DMSO and DC_517 1 μM cells in the presence of DMSO or 1 μM UC2288. * p < 0.05, ** p < 0.01, *** p < 0.001.

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