Fig. 7: DNMT1 deficiency led to increased apoptosis via activation of p53-caspase-3 pathway. | Cell Death & Differentiation

Fig. 7: DNMT1 deficiency led to increased apoptosis via activation of p53-caspase-3 pathway.

From: DNMT1 regulates human erythropoiesis by modulating cell cycle and endoplasmic reticulum stress in a stage-specific manner

Fig. 7: DNMT1 deficiency led to increased apoptosis via activation of p53-caspase-3 pathway.

A qRT-PCR results showing p53, BAX, p21 mRNA expression levels in DNMT1 deficiency erythroblasts cultured for 15 days. GAPDH was used as internal reference. B Representative western blot analysis of p53, BAX, p21. GAPDH was used as loading control. C Quantitative analysis of p53, BAX, p21 from 3 independent experiments. D Representative flow cytometry profiles of apoptosis as assessed by Annexin V and 7AAD staining of Normal-DMSO and 1 μM DC_517 cells cultured for 15 days in the presence of DMSO or 2 μM PFTα. E Quantitative analysis of apoptosis from 3 independent experiments. F Growth curves of Normal-DMSO and 1 μM DC_517 cells in the presence of DMSO or 2 μM PFTα for 7, 9, 11, 13, and 15 days. G Representative western blot analysis of Total caspase-3, cleave caspase-3. GAPDH was used as loading control. H Quantitative analysis of caspase-3, cleave caspase-3 from 3 independent experiments. I Representative flow cytometry profiles of apoptosis as assessed by Annexin V and 7AAD staining of Normal-DMSO and DC_517 1 μM cells cultured for 15 days in the presence of DMSO or 10 μM DEVD. J Quantitative analysis of apoptosis from 3 independent experiments. K Growth curves of N-DMSO and 1 μM DC_517 cells in the presence of DMSO or 10 μM DEVD for 7, 9, 11, 13 and 15 days. * p < 0.05, ** p < 0.01, *** p < 0.001.

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