Fig. 2: EGFR regulates TNF-α signalling pathways by interacting with TNFR1.
From: EGFR inhibits TNF-α-mediated pathway by phosphorylating TNFR1 at tyrosine 360 and 401
A–C HeLa cells were treated with EGF for 15 min, and then treated with TNF-α. A Heatmap showing endogenous genes upregulated by TNF-α. B Z-score of (A), (C) mRNA expression of NFKBIA, CXCL2, and CXCL3. D HeLa cells were treated with EGF for 15 min, and then treated with TNF-α for the indicated times, after which the cells were analysed by immunoblotting. E HeLa cells were treated with EGF for 15 min and then with TNF-α for the indicated times. After treatment, the cells were lysed with lysis buffer and incubated with an anti-TNFR1 antibodies. Samples were precipitated by incubation with protein G agarose, followed by immunoblotting using the indicated antibodies. F HT-29 cells were treated with Erlo for 30 min and then with TNF-α for the indicated times. After treatment, the cells were analysed using immunoblotting. G HT-29 cells were treated with Erlo for 30 min and then with TNF-α for the indicated times. After treatment, the cells were lysed with lysis buffer and incubated with an anti-TNFR1 antibodies. Samples were precipitated by incubation with protein G agarose, followed by immunoblotting using the indicated antibodies.
