Fig. 5: RIPK3 RHIM-dependent signaling promotes skin inflammation in ZBP1ca^E-het mice.

A Graph depicting lesion-free survival of ZBP1ca^E-het, ZBP1ca^E-het Ripk3^mR/mR and healthy littermates. B Graph depicting survival curve of ZBP1ca^E-het, ZBP1ca^E-het Ripk3^mR/mR and healthy littermates. C Graph depicting epidermal thickness of mice with indicated genotypes at P10–12. Mean + SEM are shown. One dot represents one mouse. Data from the same ZBP1ca^E-het mice are included in all Figures for comparison. D Representative images from abdominal and tail skin sections of ZBP1ca^E-het Ripk3^mR/mR, ZBP1ca^E-het and control mice stained with H&E (Scale bars = 100 µM, n = 5 for ZBP1ca^E-het Ripk3^mR/mR, n = 11 for ZBP1ca^E-het, n = 3 for control), CC3⁺ (n = 5 for ZBP1ca^E-het, n = 5 for ZBP1ca^E-het Ripk3^mR/mR, n = 3 for control) or immunostained with K10, K14 and K6 (Scale bars = 50 µM, n = 5 for ZBP1ca^E-het Ripk3^mR/mR, n = 11 for ZBP1ca^E-het, n = 3 for control) at P10–12. E Graph showing the amount of CC3⁺ cells in belly and tail skin of mice with indicated genotypes. Mean ± SEM are shown. Each dot represents one mouse. Data from the same ZBP1ca^E-het mice are included in all Figures for comparison. F Graphs depicting relative mRNA expression of the indicated cytokines and ISGs in RNA from whole-skin tissue of P10–12 mice of the indicated genotypes measured by qRT-PCR. Each dot represents one mouse. Mean ± SEM are shown. Data from the same ZBP1ca^E-het mice are included in all Figures for comparison. Statistical significance was determined using Kruskal–Wallis test (one-sided) in (C, E, F).