Fig. 5: SOX4 is required for brown adipocyte differentiation in vitro. | Cell Death & Differentiation

Fig. 5: SOX4 is required for brown adipocyte differentiation in vitro.

From: SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice

Fig. 5: SOX4 is required for brown adipocyte differentiation in vitro.The alternative text for this image may have been generated using AI.

A Schematic drawing showed that the immortalized BAT SVF cells were established and differentiated into mature adipocytes. B, C The immortalized BAT SVF cells infected with scrambled or shSox4 lentivirus were differentiated into mature adipocytes as indicated in Fig. 5A. On day 6 of differentiation, mature brown adipocytes were subjected to Oil Red O staining (B) and triglyceride measurement (C) (n = 3). Scale bar, 100 μm. D, E The mRNA levels of BAT-selective genes (D) and WAT-selective genes (E) in differentiated BAT adipocytes generated as in (A) (n = 3). 18S was used as an invariant control. The mRNA levels in control cells were normalized to 1.0. F The protein levels of BAT enriched proteins and AGT in mature brown adipocytes. G RT-PCR analysis of mitochondrial number markers in mature brown adipocytes (n = 3). Ppib was used as an invariant control. The mRNA levels in control cells were normalized to 1.0. H–J BAT SVF cells were infected with scrambled or shSox4 lentivirus and analyzed for Oxygen consumption rate (OCR) at day 6 of differentiation. Oligomycin, FCCP, and Rotenone / Antimycin were added at the time points indicated by the arrows and OCR was showed in (H). The averaged basal and maximal respiration rates were shown in (I) and (J), respectively (n = 3). Asterisks (*) denote the level of statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean ± SEM. Statistical analyses were determined by unpaired two-tailed Student’s t-test (C–E, G–J).

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