Fig. 3: DDI2 is required for the UPS bounce-back response during ferroptosis.
From: Activating the NFE2L1-ubiquitin-proteasome system by DDI2 protects from ferroptosis
a Immunoblot of ubiquitin in EA.hy926 cells treated with 5 µM RSL3 and 100 nM bortezomib (BTZ) for 9 h and 3 h, respectively. b Native PAGE of EA.hy926 cells treated with 10 µM NFV for 20 h and immunoblot of the ɑ1-7 (20S) subunits. c Proteasomal activity of EA.hy926 cells treated with 5 µM NFV for 9 h and 3 h of 5 µM RSL3. d Top 10 enriched gene ontology (GO) terms in the ubiquitome of DDI2 (knockout) KO compared to wild-type (WT) parental cells. e Volcano Plot of the DDI2 KO cells compared to WT cells Padj < 0.05 indicated in red. (n = 3 replicates). f Principal component analysis (PCA) of the ubiquitome of EaHy926 WT vs. DDI2 KO cells.
