Fig. 1: Endogenous BDNF and TrkB receptors are spatially associated with PLS core regions in aneural AChR clusters. | Cell Death & Differentiation

Fig. 1: Endogenous BDNF and TrkB receptors are spatially associated with PLS core regions in aneural AChR clusters.

From: Localized release of muscle-generated BDNF regulates the initial formation of postsynaptic apparatus at neuromuscular synapses

Fig. 1

Representative images (a) and quantification (b) showing the spatial localization of endogenous BDNF, proBDNF, TrkB, and pTrkB receptors at aneural AChR clusters in cultured Xenopus muscles. Arrows and arrowheads indicate localized signals at the perforated and AChR-rich regions of aneural clusters, respectively. c Representative images showing the spatial association (arrows) between BDNF and PLS at perforated aneural AChR clusters. The yellow lines indicate the region-of-interest for generating line profiles. Line profile plots showing the relative fluorescence intensities of AChR (red), BDNF (green), and PLS core and cortex markers (magenta). The colored boxes indicate the spatial enrichment of markers with their respective fluorescence signals above the cutoff intensity of 50%. d Maximal projection of Airyscan confocal z-stack images showing the spatial enrichment of BDNF (top panels) or proBDNF (bottom panels) at PLS core regions within aneural AChR clusters. The orthogonal xz views show the differential distributions of AChR, cortactin, and BDNF/proBDNF. Scale bars represent 5 µm, unless stated otherwise.

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