Fig. 2: Overexpression of AKR1C1 is correlated with ECC progression and poor prognosis. | Cell Death & Differentiation

Fig. 2: Overexpression of AKR1C1 is correlated with ECC progression and poor prognosis.

From: The AKR1C1–CYP1B1–cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma

Fig. 2

A qRT-PCR analysis of AKR1C1 expression in 55 pairs of human primary ECC tissues and adjacent non-tumor tissues. GAPDH expression was used as an internal control for normalization. N normal tissues, T tumor tissues. B Representative IHC staining of AKR1C1 in normal extrahepatic bile duct tissue and different histologic grade of human ECC specimens (n = 55), including well differentiated, moderately differentiated, and poorly differentiated. Statistical analysis results are shown on the right, based on quantification of the percentage of AKR1C1+ area in each tissue section. WD well differentiation, MD moderate differentiation, PD poor differentiation. C Dot distribution graph of AKR1C1 IHC staining scores was shown in 55 ECC patients of different clinical stages. D Correlation between AKR1C1 protein expression and tumor diameter (n = 55). E Correlation between AKR1C1 protein expression and lymph node metastasis (n = 55). F Kaplan–Meier analysis of overall survival and G recurrence-free survival based on AKR1C1 expression in 55 ECC patients. Data are presented as the mean ± standard deviation from three independent experiments, n.s. > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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