Fig. 1: Characterization of palbociclib-induced senescent melanoma cells. | Cell Death & Differentiation

Fig. 1: Characterization of palbociclib-induced senescent melanoma cells.

From: HRK downregulation and augmented BCL-xL binding to BAK confer apoptotic protection to therapy-induced senescent melanoma cells

Fig. 1: Characterization of palbociclib-induced senescent melanoma cells.The alternative text for this image may have been generated using AI.

A Quantification of SA-β-galactosidase in proliferating and palbociclib-induced senescent melanoma cells (senescent-P) by flow cytometry. B % of p21 and p16 in proliferating and palbociclib-induced senescent (senescent-P) melanoma cells measured by flow cytometry. C, D Upper panels: Representative images of transmission electron microscopy from proliferating and palbociclib-induced senescent (senescent-P) melanoma cells. ER endoplasmic reticulum, ly lysosome, mit mitochondria, LD lipid droplet, g Golgi complex, Nu nucleus. Scale bar: 1 μm. Lower panels: Quantification of mitochondrial circularity and the number of lysosomes in proliferating and palbociclib-induced senescent melanoma cells. Values indicate mean values ± SEM from at least three independent experiments. ****p < 0.0001, *** p < 0.001, **p < 0.01, *p < 0.05 compared to control.

Back to article page