Fig. 8: Localized methionine deprivation in the bladder promotes immune cell infiltration and inhibits PD-L1 expression. | Cell Death & Differentiation

Fig. 8: Localized methionine deprivation in the bladder promotes immune cell infiltration and inhibits PD-L1 expression.

From: Methionine-driven YTHDF1 expression facilitates bladder cancer progression by attenuating RIG-I-modulated immune responses and enhancing the eIF5B-PD-L1 axis

Fig. 8: Localized methionine deprivation in the bladder promotes immune cell infiltration and inhibits PD-L1 expression.

A Schematic of the mouse experiment. B Tumor growth curves of mice in different treatment groups. C ELISA detection of methionine levels in the peripheral blood of mice from different treatment groups. ELISA detection of methionine (D) and IFN-β (E) levels in the tumors of mice from different treatment groups. F Representative histograms and bar graphs showing the differences in PD-L1 expression in tumor cells across different treatment groups. G Representative flow cytometry plots and bar graphs showing the proportions of tumor-infiltrating CD8+ T cells per gram of tumor tissue in each treatment group. H Representative flow cytometry plots and bar graphs showing the proportions of SLAMF6− and CXCR6+ CD8+ T cells in the tumors of each treatment group. Immunofluorescence images of CD8, YTHDF1, RIG-I, PD-L1, and DAPI staining in bladder cancer patients who responded (I) or were resistant (J) to immunotherapy from our center. K–N Bar graphs showing the statistical differences in immunofluorescence staining results between patients with different responses to immunotherapy. ns, P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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