Fig. 4: YAPer-ORF competes with PRP4K to dephosphorylate and sequester YAP in the nucleus. | Cell Death & Differentiation

Fig. 4: YAPer-ORF competes with PRP4K to dephosphorylate and sequester YAP in the nucleus.

From: The LINC01315-encoded small protein YAPer-ORF competes with PRP4k to hijack YAP signaling to aberrantly promote cell growth

Fig. 4: YAPer-ORF competes with PRP4K to dephosphorylate and sequester YAP in the nucleus.The alternative text for this image may have been generated using AI.

a Structural comparison of YAPer-ORF-PRP4K via AlphaFold2. b Coimmunoprecipitation (co-IP) assays showing the interactions of PRP4K with endogenous YAP and ectopic ORF proteins. c Co-IP assays showing interactions of ectopic ORF with endogenous YAP and PRP4K proteins. d An in vitro kinase assay was used to assess the kinase activity of PRP4K with/without YAPer-ORF. Co-IP assays (e) and quantification (f) showing interactions of ectopic YAP with endogenous PRP4K proteins (upper); Co-IP assays showing interactions of endogenous PRP4K with ectopic ORF proteins (lower).

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