Fig. 1: Analysis of proteomics and transcriptomics in activated macrophages.

A Pattern diagram of proteomics and transcriptomics analysis in macrophages with or without inflammatory stimulation. B GO functional enrichment analysis of differentially expressed genes (DEGs) of transcriptomics in BMDMs with or without LPS-V treatment (1 μg/ml, 2 h). C Volcano map showing proteomic differentially expressed genes in BMDMs with or without LPS-V treatment (1 μg/ml, 2 h). Red indicates highly expressed proteins by LPS-V; Blue indicates low expression proteins by LPS-V; The circles represent proteins that were inconsistent with RNA sequencing; Key proteins are labeled by the protein names. D Venn diagram shows the number of identical or different genes for differentially expressed genes as determined by RNA sequencing and quantitative proteomics. RNA down, RNA up, Protein down and Protein up represent low expression in RNA seq, high expression in RNA seq, low expression in proteome, and high expression in proteome induced by LPS-V, respectively. E GO functional enrichment analysis of the differentially expressed proteins whose correspondent mRNA levels don’t show the same alteration. LPS treatments decrease the protein expression of DPEP2 but not DPEP1 or DPEP3 in BMDMs (F) and human macrophages (G). LPS treatments have no significant influence on the mRNA expression of DPEP1/2/3 in BMDMs (H) and human macrophages (I). All data are expressed as mean ± SD. ns denotes no signification.