Fig. 2: DPEP2 is upregulated in the macrophages.

A t-SNE diagram shows 13 cell types sorted by single cell transcriptome sequencing in bone marrow tissue provided by two healthy people. B Dot Plot showed the expression of the first 2 marker genes of 13 cell types and the gene of interest DPEP2 in each cell type. C GMP- Monocyte-macrophage differentiation locus. D The expression of DPEP2 increased gradually during the differentiation from GMPs to macrophages. Calculation of DPEP2 expression levels of different cell subpopulations in ovarian tumor tissues (E) and colorectal carcinoma tissues (F) using the TISCH2 Dataset. The expression of DPEP1/2/3 protein (G) and mRNA (H) during the differentiation of BMDM from HSCs in vitro. I The expression of DPEP1/2/3 protein detected by Western blot assays during the differentiation of human macrophages in vitro. J The expression of DPEP1/2/3 protein detected by Western Blot assays during the differentiation of macrophages from THP-1 by PMA treatment in vitro. K The expression of DPEP2 protein detected by Western blot assays in BMDMs from WT or DPEP2-KO HSCs in vitro. L The relative differentiation efficiency of BMDMs from WT or DPEP2-KO HSCs in vitro. DPEP2 knockout has no influences on the cell vitality of THP-1 (M) or iBMDMs (N). All data are expressed as mean ± SD. ns denotes no signification. ***P < 0.001.