Fig. 7: Inhibition of Trim32 represses transduction of NF-κB and p38 signalings by DPEP2-mediated MAP3K7 inactivation. | Cell Death & Differentiation

Fig. 7: Inhibition of Trim32 represses transduction of NF-κB and p38 signalings by DPEP2-mediated MAP3K7 inactivation.

From: The Trim32-DPEP2 axis is an inflammatory switch in macrophages during intestinal inflammation

Fig. 7: Inhibition of Trim32 represses transduction of NF-κB and p38 signalings by DPEP2-mediated MAP3K7 inactivation.

A Ubiquitination levels of Trim32 were increased in colonic macrophages from mouse model of IBD, GVHD, and HAEC like intestinal inflammation. B–E Trim32 increases the interaction of MAP3K7 with DPEP2 and decreases interaction of MAP3K7 with IKKβ and p38 by increasing the expression of DPEP2 in macrophages with LPS. Co-IP assays detecting the interaction of MAP3K7 and p38 or IKKβ were in (B). PLA assays of interaction of MAP3K7 and DPEP2 were in (C). n = 300 per group. PLA assays of interaction of MAP3K7 and p38 were in (D). n = 300 per group. PLA assays of interaction of MAP3K7 and IKKβ were in (D). n = 300 per group. The effects of RING-deficiency of Trim32 on the interaction of MAP3K7 with IKKβ and p38 in the WT (F) or DPEP2-KO (G) iBMDMs. All data are expressed as mean ± SD. **P < 0.01, and ***P < 0.001.

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