Fig. 8: Inhibition of Trim32 represses development of inflammation by DPEP2 in vivo.
From: The Trim32-DPEP2 axis is an inflammatory switch in macrophages during intestinal inflammation
A Pattern diagram of IBD and GVHD models with Trim32 KD BM cells. B6-background BM cells transfected with a conditional shRNA system targeting Trim32 were transplanted into irradiated myeloablative mice. IBD and GVHD models were established using recipient mice to detect the role of Trim32. The image was created by biorender.com. B Trim32 knockdown using a conditional shRNA system in BM cells with or without Dox treatment. Trim32 KD in HSCs decreases the expression of serum and colonic proinflammatory cytokines in IBD models (C, E, n = 4 per group), which is blocked by DPEP2 deletion (D, F, n = 4 per group). Trim32 KD in HSCs decreases the expression of serum and colonic proinflammatory cytokines in GVHD models (G, I, n = 4 per group), which is blocked by DPEP2 deletion (H, J, n = 4 per group). All data are expressed as mean ± SD. *P < 0.05, ns denotes no signification.
